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4495175 Preparation of highly purified human antihemophilic factor


Book ID
123367197
Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
74 KB
Volume
3
Category
Article
ISSN
0734-9750

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Rapid preparation of highly purified hum
โœ Neil D. Cook; Robert J. Simpson; Kurt Osterloh; Timothy J. Peters ๐Ÿ“‚ Article ๐Ÿ“… 1985 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 761 KB

A rapid, two-step purification for human transfenin using DEAE-Sephacel chromatography followed by phenyl-boronate affinity chromatography is described. The method gives a 70% yield and isolates transferrin of over 97% purity which is hemopexin-free. The remaining protein is due to a single contamin