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Preparation of highly purified F-actin-depolymerizing factor of human serum

✍ Scribed by Rigmor THORSTENSSON; Catharina STERKY; Renée NORBERG


Book ID
114675322
Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
728 KB
Volume
147
Category
Article
ISSN
1432-1327

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A rapid, two-step purification for human transfenin using DEAE-Sephacel chromatography followed by phenyl-boronate affinity chromatography is described. The method gives a 70% yield and isolates transferrin of over 97% purity which is hemopexin-free. The remaining protein is due to a single contamin