Rapid preparation of highly purified hum
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Neil D. Cook; Robert J. Simpson; Kurt Osterloh; Timothy J. Peters
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Article
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1985
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Elsevier Science
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English
β 761 KB
A rapid, two-step purification for human transfenin using DEAE-Sephacel chromatography followed by phenyl-boronate affinity chromatography is described. The method gives a 70% yield and isolates transferrin of over 97% purity which is hemopexin-free. The remaining protein is due to a single contamin