Rapid preparation of highly purified human transferrin

✍ Scribed by Neil D. Cook; Robert J. Simpson; Kurt Osterloh; Timothy J. Peters

• Book ID: 102984919
• Publisher: Elsevier Science
• Year: 1985
• Tongue: English
• Weight: 761 KB
• Volume: 149
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(85)90581-0

✦ Synopsis

A rapid, two-step purification for human transfenin using DEAE-Sephacel chromatography followed by phenyl-boronate affinity chromatography is described. The method gives a 70% yield and isolates transferrin of over 97% purity which is hemopexin-free. The remaining protein is due to a single contaminant which may be the glycosylated form of albumin.