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1H NMR detection of vitamin C in human brain in vivo

✍ Scribed by Melissa Terpstra; Rolf Gruetter

Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
138 KB
Volume
51
Category
Article
ISSN
0740-3194

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✦ Synopsis

Abstract

Vitamin C (ascorbate) is well established as an essential nutrient that functions as an antioxidant. Since it is present in the human brain at detectable concentrations, this study was designed to detect and quantify ascorbate in the human brain in vivo using ^1^H NMR spectroscopy (MRS). Ascorbate was consistently detected in all five study subjects, and was measured using MEGA‐PRESS difference editing. The in vivo resonance pattern was consistent with that of ascorbate based on position, line width, peak pattern, and relative intensity. Metabolites with a potential for coediting were assessed using phantom solutions. The putative resonances of myo‐inositol, lactate, glycerophosphocholine, phosphocholine, and phosphoethanolamine were detected at positions distinct from those of ascorbate. This study represents the first in vivo detection of vitamin C in the human brain using ^1^H MRS. A concentration of 1.3 ± 0.3 μmol/g (mean ± SD, N = 4) was estimated. Magn Reson Med 51:225–229, 2004. © 2004 Wiley‐Liss, Inc.

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