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Localized in vivo 1H NMR detection of neurotransmitter labeling in rat brain during infusion of [1-13C] D-glucose

✍ Scribed by Josef Pfeuffer; Ivan Tkáč; In-Young Choi; Hellmut Merkle; Kâmil Uǧurbil; Michael Garwood; Rolf Gruetter

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
170 KB
Volume
41
Category
Article
ISSN
0740-3194

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✦ Synopsis

Resolved localized nuclear magnetic resonance (NMR) signals of 1 H bound to 13 C label in the carbon positions of glutamate C4, C3 and glutamine C4, C3, as well as in aspartate C3, lactate C3, alanine C3, ␥-aminobutyric acid C3, and glucose C1 were simultaneously observed in spectra obtained from rat brain in vivo. Time-resolved label incorporation was measured with a new adiabatic carbon editing and decoupling (ACED) singlevoxel stimulated echo acquistion mode (STEAM) sequence. Adiabatic carbon broadband decoupling of 12 kHz bandwidth was achieved in vivo, which decoupled the entire 13 C spectrum at 9.4 T. Resonances from N-acetyl-aspartate and creatine were also detected, consistent with natural-abundance 13 C levels. These results emphasize the potential of 1 H NMR for following complex biochemical pathways in localized areas of resting rat brain as well as during focal activation using infusions of 13 C-labeled glucose. Magn Reson Med 41:

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