1 H− 31 P CPMG-Correlated Experiments for the Assignment of Nucleic Acids
✍ Scribed by Luy, Burkhard; Marino, John P.
- Book ID
- 127267756
- Publisher
- American Chemical Society
- Year
- 2001
- Tongue
- English
- Weight
- 42 KB
- Volume
- 123
- Category
- Article
- ISSN
- 0002-7863
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A three-dimensional heteronuclear NMR technique to correlate 31 P spins with 1 H along the backbone of DNA is described. The approach is based on a 31 P-1 H heteronuclear long-range correlation (HELCO) experiment in its refocused form. The utility of this technique is demonstrated by the sequence-sp
13 C-labeled but the nucleic acid is not, 12 C-filtering is readily achieved by the application of a series of 13 C purge pulses during the constant time evolution period without any loss of signal-to-noise of the nucleic acid cross peaks. The method is demonstrated for the Dickerson DNA dodecamer a