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Determination of Three-Bond1H3′–31P Couplings in Nucleic Acids and Protein–Nucleic Acid Complexes by QuantitativeJCorrelation Spectroscopy

✍ Scribed by G.Marius Clore; Elizabeth C. Murphy; Angela M. Gronenborn; Ad Bax

Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
92 KB
Volume
134
Category
Article
ISSN
1090-7807

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✦ Synopsis

13 C-labeled but the nucleic acid is not, 12 C-filtering is readily achieved by the application of a series of 13 C purge pulses during the constant time evolution period without any loss of signal-to-noise of the nucleic acid cross peaks. The method is demonstrated for the Dickerson DNA dodecamer and a 19 kDa complex of the transcription factor SRY with a 14mer DNA duplex. The same approach should be equally applicable to numerous other problems, including the measurement of J H-Cd couplings in cadmium-ligated proteins, or 3 J CH couplings in other selectively enriched compounds.

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