Fine Structure of the P–H5′ Cross-Peak in 31P–1H Correlated 2D NMR Spectroscopy. An Efficient Probe for the Backbone Torsion Angles β and γ in Nucleic Acids
✍ Scribed by Jeroen A. Pikkemaat; Cornelis Altona
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 701 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0749-1581
No coin nor oath required. For personal study only.
✦ Synopsis
The splitting pattern of the P-H5" (and P-H5') cross-peak in a 2D 31P-1H correlated NMR spectrum is analysed. Since this cross-peak pattern is strongly dependent on two coupling constants, 'J(PH5") and 3J(H4H5"), it is an efficient probe for the backbone torsion angles fl and y in nucleic acids. A method, based on cross-peak simulation, is described to determine the values of 3J(PH5") and 3J(H4'H5'') in a semiquantitative fashion from the fine structure of the multiplet. The method is exempliied by the analysis of the P-H5" cross peaks of the guanine residues at the 3' + 5' loopstem junctions of two dumbbell-shaped DNA double-hairpin loop structures.