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31P–1H correlation and resonance assignments along the DNA backbone: three-dimensional implementation of heteronuclear long-range correlation experiment

✍ Scribed by Vinit K. Rastogi

Publisher: John Wiley and Sons
Year: 2000
Tongue: English
Weight: 91 KB
Volume: 38
Category: Article
ISSN: 0749-1581
DOI: 10.1002/(sici)1097-458x(200004)38:4<288::aid-mrc636>3.0.co;2-s

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✦ Synopsis

A three-dimensional heteronuclear NMR technique to correlate 31 P spins with 1 H along the backbone of DNA is described. The approach is based on a 31 P-1 H heteronuclear long-range correlation (HELCO) experiment in its refocused form. The utility of this technique is demonstrated by the sequence-specific resonance assignment of 31 P and 1 H spins along the backbone of a mismatched base-paired dodecanucleotide, d-GGTACIAGTACC.

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✍ M. H. Frey; W. Leupin; O. W. Sørensen; W. A. Denny; R. R. Ernst; K. Wüthrich 📂 Article 📅 1985 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 534 KB

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