✦ LIBER ✦

Sequence-specific assignment of the backbone 1H- and 31P-nmr lines in a short DNA duplex with homo- and heteronuclear correlated spectroscopy

✍ Scribed by M. H. Frey; W. Leupin; O. W. Sørensen; W. A. Denny; R. R. Ernst; K. Wüthrich

Publisher: Wiley (John Wiley & Sons)
Year: 1985
Tongue: English
Weight: 534 KB
Volume: 24
Category: Article
ISSN: 0006-3525
DOI: 10.1002/bip.360241214

No coin nor oath required. For personal study only.

✦ Synopsis

We report the assignment of the backbone 'Hand 31P-nmr lines in the synthetic hexadeoxyribonucleotide pentaphosphate duplex d(GCATGC),, using double quantum filtered 'H-lH correlation spectroscopy, 'H observed IH3P heteronuclear correlation spectroscopy, and 31P relayed 'H-IH correlation spectroscopy. The strategy used enables one to make sequence-specific resonance assignments without reference to a known or assumed conformation of the DNA fragment.

📜 SIMILAR VOLUMES

Backbone Conformational Study of a Non-P

Backbone Conformational Study of a Non-Palindromic 16 Base Pair DNA Duplex Exploring 2D 31P–1H Heteronuclear Inverse Spectroscopy: Assignment of all NMR Phosphorus Resonances and Measurement of 3J31P–1H3′ Coupling Constants

✍ Carine Tisne; Catherine Simenel; Edith Hantz; Francis Schaeffer; Muriel Delepier 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 884 KB

Assignment of all 31P resonances of a 16 base-pair DNA duplex, Sd(CTGCTCACTTTCCAGG)Y Sd(CCTGGAAAGTGAGCAG)Y, related to the DNA KB site of the HIV-1 LTR, was determined by 2D heteronuclear inverse NMR spectroscopy (HSQC-TOCSY and heteronuclear COSY). 'J(H3'-P) coupling constants for most of the oligo