Backbone Conformational Study of a Non-Palindromic 16 Base Pair DNA Duplex Exploring 2D 31P–1H Heteronuclear Inverse Spectroscopy: Assignment of all NMR Phosphorus Resonances and Measurement of 3J31P–1H3′ Coupling Constants
✍ Scribed by Carine Tisne; Catherine Simenel; Edith Hantz; Francis Schaeffer; Muriel Delepierre
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 884 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0749-1581
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✦ Synopsis
Assignment of all 31P resonances of a 16 base-pair DNA duplex, Sd(CTGCTCACTTTCCAGG)Y Sd(CCTGGAAAGTGAGCAG)Y, related to the DNA KB site of the HIV-1 LTR, was determined by 2D heteronuclear inverse NMR spectroscopy (HSQC-TOCSY and heteronuclear COSY). 'J(H3'-P) coupling constants for most of the oligomer phosphates were obtained using heteronuclear selective COSY. Theoretical considerations of the 'P relaxation in macromolecules in solution allow parameter optimization of two-dimensional 'H-"P NMR experiments. Structural information can then be extracted to define the backbone conformation of large oligonucleotides in solution.