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μ-type calcium-activated neutral protease in the rat peripheral nerve

✍ Scribed by K. Kamakura; S. Ishiura; H. Sugita

Publisher
John Wiley and Sons
Year
1986
Tongue
English
Weight
703 KB
Volume
15
Category
Article
ISSN
0360-4012

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✦ Synopsis

Previously we reported results of an incubation experiment with neurofilaments that supported the existence of a p-type of Ca2+-activated neutral protease in the rat peripheral nerve; it was active with pM order Ca2' (p-CANP). This time, we partially purified the p-CANP from a crude CANP fraction of rat peripheral nerve by using a DE52 column and a Phenyl-Sepharose column followed again by DE52 column chromatography. The presence of p-CANP was verified by an immunoblotting technique. The p-CANP degraded the neurofilament triplet as previously reported; i.e., among the neurofilament triplet, the 160K component was most sensitive, and in the order of the 160K, 68K, and 200K components, respectively.

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