The correlation between calcium absorption and electrophysiological recovery in crushed rat peripheral nerves

✍ Scribed by Ji-Geng Yan; Hani S. Matloub; Yuhui Yan; Michael Agresti; Lin-Ling Zhang; Safwan S. Jaradeh

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 510 KB
• Volume: 30
• Category: Article
• ISSN: 0738-1085
• DOI: 10.1002/micr.20709

✦ Synopsis

Abstract

The correlation between calcium ion (Ca^2+^) concentration and electrophysiological recovery in crushed peripheral nerves has not been studied. Observing and quantifying the Ca^2+^ intensity in live normal and crushed peripheral nerves was performed using a novel microfine tearing technique and Calcium Green‐1 Acetoxymethyl ester stain, a fluorescent Ca^2+^ indicator. Ca^2+^ was shown to be homogeneously distributed in the myelinated sheaths. After a crush injury, there was significant stasis in the injured zone and the portion distal to the injury. The Ca^2+^ has been almost completely absorbed after 24 weeks in the injured nerve to be similar to the controls. The process of the calcium absorption was correlated with the Compound Muscle Action Potential recovery process of the injured nerves. This correlation was statistically significant (r = −0.81, P < 0.05). The better understanding of this process will help us to improve nerve regeneration after peripheral nerve injury. © 2009 Wiley‐Liss, Inc. Microsurgery, 2010.