## Abstract Die spezifische Aktivität der aus __Candida guilliermondii__, Stamm H 17, nach Anzucht auf Acetat als C‐Quelle gewonnenen Isocitratlyase konnte durch Ammoniumsulfatfällung (60–75%) und Gelchromatographie (Sephadex G 200) 3,7 fach angereichert werden. Die gereinigten Enzymfraktionen wur
Zellelektrophoretische Charakterisierung der Oberfläche von Candida guilliermondii
✍ Scribed by Dr. K.-H. Lerche; G. Kretzschmar
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 794 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0233-111X
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
The electrical properties of the outer layer of the cell wall of a hydrocarbon‐grown yeast C. guilliermondii were studied in detail by cell electrophoresis. Some experiments were also made with the yeast S. cerevisiae. The mobilities of the yeasts were measured as a function of pH at pH 2‐11.5 and constant ionic strength I = 0.02 mol/1. For identification of surface groups the pH‐mobility curves are used to calculate pK‐values. The results indicate the presence of amino and carboxyl groups on the surface of C. guilliermondii. These groups are probably a part of a glycoprotein with an isoelectric point at pH 3.3 located on the surface. Electrophoresis of droplets of mineral oil coated with adsorbed protein layer confirms these conclusions. The importance of this glycoprotein in hydrocarbon uptake is discussed.
📜 SIMILAR VOLUMES
## Abstract Nach dem Entleeren von Flüssigkeiten aus Gefäßen oder Leitungen verbleibt stets ein Rest an den Wandungen. Für die Ausbildung eines solchen Flüssigkeitsfilmes an der Oberfläche eines Werkstoffes sind die chemische Natur des Materials selbst, die physikalische Beschaffenheit der Oberfläc
The DNA content of the nucleus of the yeast Candida rec. EH 15, Lodderomyces elongisporus CBS 2605, Pichia guillermondii SO809, Pichia guilliermondii fpl-61, and Saccharomyces cerevisiae D10 has been determined by renaturation kinetics. It was possible to make conclusions for the ploidy by measureme