X-ray microanalysis of fluid spaces in the frozen cochlea
β Scribed by I. Hunter-Duvar; J. Landolt; R. Cameron
- Book ID
- 112312058
- Publisher
- Springer-Verlag
- Year
- 1981
- Tongue
- English
- Weight
- 645 KB
- Volume
- 230
- Category
- Article
- ISSN
- 0302-9530
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## Abstract Elemental microanalysis of biological material in the frozenβhydrated state using inβvacuum proton induced Xβray emission is described for the first time. For this purpose, a commercially available cryotransfer system was modified and coupled to the experimental chamber of the nuclear m
The analysis of bulk frozen-hydrated biological samples has developed now to a level where practical application of the technique is possible. Provided the sample is carefully coated with a conductive metal, the development of a space charge capable of causing a significant distortion of the electro