The preparation of frozen-hydrated tissue sections for X-ray microanalysis
β Scribed by A. Saubermann; P. Echlin
- Book ID
- 115848713
- Publisher
- Elsevier Science
- Year
- 1974
- Tongue
- English
- Weight
- 109 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0011-2240
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The analysis of bulk frozen-hydrated biological samples has developed now to a level where practical application of the technique is possible. Provided the sample is carefully coated with a conductive metal, the development of a space charge capable of causing a significant distortion of the electro
## Abstract Elemental microanalysis of biological material in the frozenβhydrated state using inβvacuum proton induced Xβray emission is described for the first time. For this purpose, a commercially available cryotransfer system was modified and coupled to the experimental chamber of the nuclear m