X-ray imaging of various biological samples using a phase-contrast hard X-ray microscope
โ Scribed by Guk Bae Kim; Yae Jin Yoon; Tae Joo Shin; Hwa Shik Youn; Yong Song Gho; Sang Joon Lee
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 295 KB
- Volume
- 71
- Category
- Article
- ISSN
- 1059-910X
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โฆ Synopsis
Abstract
In this study, we visualized the internal structures of various bioโsamples and found the optimum conditions of test samples for the 7 keV hard Xโray microscope of the Pohang light source. From the captured Xโray images, we could observe the intercellular and intracellular structures of dehydrated human cells and mouse tumor tissues without using any staining materials in a spatial resolution better than 100 nm. The metastasized lung tissue, which was several tens of micrometers in thickness, was found to be very well suited to this hard Xโray microscope system, because it is nearly impossible to observe such a nontransparent and thick sample with a high spatial resolution better than 100 nm using any microscopes such as a soft Xโray microscope, an optical microscope, or an electron microscope. Microsc. Res. Tech., 2008. ยฉ 2008 WileyโLiss, Inc.
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