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X-ray imaging of various biological samples using a phase-contrast hard X-ray microscope

โœ Scribed by Guk Bae Kim; Yae Jin Yoon; Tae Joo Shin; Hwa Shik Youn; Yong Song Gho; Sang Joon Lee


Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
295 KB
Volume
71
Category
Article
ISSN
1059-910X

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โœฆ Synopsis


Abstract

In this study, we visualized the internal structures of various bioโ€samples and found the optimum conditions of test samples for the 7 keV hard Xโ€ray microscope of the Pohang light source. From the captured Xโ€ray images, we could observe the intercellular and intracellular structures of dehydrated human cells and mouse tumor tissues without using any staining materials in a spatial resolution better than 100 nm. The metastasized lung tissue, which was several tens of micrometers in thickness, was found to be very well suited to this hard Xโ€ray microscope system, because it is nearly impossible to observe such a nontransparent and thick sample with a high spatial resolution better than 100 nm using any microscopes such as a soft Xโ€ray microscope, an optical microscope, or an electron microscope. Microsc. Res. Tech., 2008. ยฉ 2008 Wileyโ€Liss, Inc.


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