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Why do crown ethers activate enzymes in organic solvents?

✍ Scribed by Dirk-Jan van Unen; Johan F. J. Engbersen; David N. Reinhoudt


Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
122 KB
Volume
77
Category
Article
ISSN
0006-3592

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The dramatic activation of serine proteases in nonaqueous media resulting from lyophilization in the presence of KCl is shown to be unrelated to relaxation of potential substrate diffusional limitations. Specifically, lyophilizing subtilisin Carlsberg in the presence of KCl and phosphate buffer in d