The dramatic activation of serine proteases in nonaqueous media resulting from lyophilization in the presence of KCl is shown to be unrelated to relaxation of potential substrate diffusional limitations. Specifically, lyophilizing subtilisin Carlsberg in the presence of KCl and phosphate buffer in d
Salt-activation of nonhydrolase enzymes for use in organic solvents
β Scribed by John A. Morgan; Douglas S. Clark
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- English
- Weight
- 89 KB
- Volume
- 85
- Category
- Article
- ISSN
- 0006-3592
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π SIMILAR VOLUMES
The addition of simple inorganic salts to aqueous enzyme solutions prior to lyophilization results in a dramatic activation of the dried powder in organic media relative to enzyme with no added salt. Activation of both the serine protease subtilisin Carlsberg and lipase from Mucor javanicus resultin
The water activities (a w ) of 13 salt hydrate pairs equilibration to occur through the gas phase. However, were determined from vapor pressure measurements; a w keeping a w constant during longer term reactions in values for a subset were also estimated from a study of which water is a reactant or
## Abstract A combinatorial experimental technique was used to identify salts and salt mixtures capable of activating penicillin amidase in organic solvents for the transesterification of phenoxyacetate methyl ester with 1βpropanol. Penicillin amidase was lyophilized in the presence of various chlo