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Whole plasmid mutagenic PCR for directed protein evolution

โœ Scribed by Ichiro Matsumura; Lori A. Rowe


Book ID
108069159
Publisher
Elsevier Science
Year
2005
Tongue
English
Weight
328 KB
Volume
22
Category
Article
ISSN
1389-0344

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Overexpressed proteins are often insoluble, and can be recalcitrant to conventional solubilization techniques such as refolding. Directed evolution methods, in which protein diversity libraries are screened for soluble variants, offer an alternative route to obtaining soluble proteins. Recently, sev