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Multiplex-PCR-Based Recombination as a Novel High-Fidelity Method for Directed Evolution

โœ Scribed by Thorsten Eggert; Susanne Aileen Funke; Nalam M. Rao; Priyamvada Acharya; Holger Krumm; Manfred T. Reetz; Karl-Erich Jaeger


Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
284 KB
Volume
6
Category
Article
ISSN
1439-4227

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Multiplex PCR-based real-time invader as
โœ Naoya Hosono; Michiaki Kubo; Yumiko Tsuchiya; Hiroko Sato; Takuya Kitamoto; Susu ๐Ÿ“‚ Article ๐Ÿ“… 2008 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 605 KB

We report the development of a real-time Invader assay combined with multiplex PCR (mPCR-RETINA), an SNP-based approach that can measure the allelic ratio in copy number variation (CNV) regions of a genome. RETINA monitors the real-time fluorescence intensity of each allele during the Invader assay