𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Improvement of PCR reaction conditions for site-directed mutagenesis of big plasmids

✍ Scribed by Bogdan Munteanu; Mario Braun; Kajohn Boonrod

Book ID
119923289
Publisher
SP Zhejiang University Press
Year
2012
Tongue
English
Weight
392 KB
Volume
13
Category
Article
ISSN
1673-1581

No coin nor oath required. For personal study only.

πŸ“œ SIMILAR VOLUMES

Isopentenyl diphosphate isomerase. Site-
Isopentenyl diphosphate isomerase. Site-directed mutagenesis of Cys139 using β€œcounter” PCR amplification of an expression plasmid
✍ Ian P. Street; Hazel R. Coffman; C.Dale Poulter πŸ“‚ Article πŸ“… 1991 πŸ› Elsevier Science 🌐 French βš– 455 KB

Sitedirected mutaQenesfs of Cys139 in yeast isopentenyf diphosphate isomerase was accomplished by "counter" pofymerase chain reactfon ampfiffcatfon of the gene in expression pfasmfd pips241 using only two DNA primers. One primer complemented a region of the sense strand and contained the mutations.