Western Blot of Proteins from Coomassie-Stained Polyacrylamide Gels
โ Scribed by Velvizhi Ranganathan; Prabir K. De
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 76 KB
- Volume
- 234
- Category
- Article
- ISSN
- 0003-2697
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A method is described for extracting proteins and peptides from stained sodium dodecyl sulfate-polyacrylamide gels. Coomassie blue and sodium dodecyl sulfate present in stained gel sections are removed to allow subsequent analysis of the peptides (e.g., amino acid analysis or tryptic digestion and f
A procedure which allows quantitative determination of the amino acid composition of stained proteins separated by sodium dodecyl sulfate (SDS)-gel electrophoresis using ninhydrin is described. The Coomassie-protein-SDS complex extracted from the gel is dialyzed, lyophylized, and subjected to amino
## Quantification of Coomassie Blue Stained Proteins in Polyacrylamide Gels Based on Analyses of Eluted Dye1 Quantification of Coomassie Brilliant Blue R-250 stained proteins on cellulose acetate strips was made over a limited range of protein concentrations (1); this work extended to quantificati