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Voltage Gradient Electrophoresis of Nucleic Acids on Agarose Gels

✍ Scribed by R. Barbieri; V. Izzo; M.A. Costa; G. Giudice; G. Duro

Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
378 KB
Volume
212
Category
Article
ISSN
0003-2697

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✦ Synopsis

A very simple method is described which allows the separation of DNA molecules in a wide molecular weight range (from 0.6 to about (30 \mathrm{~kb}) ) in the same electrophoresis agarose gel. This is based on the achievement of a voltage gradient through a simple device consisting of a Plexiglas plate placed slantwise with respect to the gel surface plane, submerged in the electrophoretic running buffer. Further applications of our system are also described. c 1993 Academic Press, Inc.

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We describe a procedure for the electroelution of nucleic acids from agarose or acrylamide gels. The method employs a combination of low-and high-ionic-strength buffers so that nucleic acids electroelute under a high-voltage gradient but accumulate over a high-salt barrier. By this means electroelut