Agarose gel electrophoresis of bacteriophages and related particles. III. Dependence of gel sieving on the agarose preparation
β Scribed by Dr. Philip Serwer; Jerry L. Allen; Shirley J. Hayes
- Publisher
- John Wiley and Sons
- Year
- 1983
- Tongue
- English
- Weight
- 536 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
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By use of agarose gel electrophoresis, the sieving of spherical particles in agarose gels has been quantitated and modeled for spheres with a radius (R) between 13.3 and 149 nm. For quantitation, the electrophoretic mobility has been determined as a function of agarose percentage (A). Because a prev
## DNA electrophoresis in agarose gels: A simple relation describing the length dependence of mobility Electrophoretic mobilities of DNA molecules ranging in length from 100 to 10 000 base pairs (bp) were measured in gels of eleven concentrations of agarose from 0.5 to 1.5%. Excellent fits of the