Voltage-dependent ATP-sensitive potassium channels of skeletal muscle membrane
โ Scribed by Spruce, A. E.; Standen, N. B.; Stanfield, P. R.
- Book ID
- 109740015
- Publisher
- Nature Publishing Group
- Year
- 1985
- Tongue
- English
- Weight
- 381 KB
- Volume
- 316
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/316736a0
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๐ SIMILAR VOLUMES
The inside-out mode of the patch-clamp technique was used to study adenosine-5'-triphosphate (ATP)-sensitive K+ channels in mammalian skeletal muscle. Vanadate, applied to the cytoplasmic face of excised patches, was a potent activator of ATP-sensitive K+ channels. Divalent cations (Mg2+, Ca2+) were
Patch-clamp techniques were used to study the effects of internal nucleotide diphosphates on the KAT e channel in mouse skeletal muscle. In inside-out patches, application of GDP (100 gM) and ADP (100 gM) reversibly increased the channel activity. In the presence of internal Mg 2+ (1 mM), low concen
We have examined the effects of 4-aminopyridine (4-AP) on single ATP-dependent potassium channels in patches excised from frog skeletal muscle. 4-AP applied to the internal face of the membrane caused a flickery block. We could not detect any flickery block when 10 mM 4-AP was applied to the externa