Modulation by Mg2+and ADP of ATP-sensitive potassium channels in frog skeletal muscle
β Scribed by Cyrille Forestier; Michel Vivaudou
- Publisher
- Springer
- Year
- 1993
- Tongue
- English
- Weight
- 734 KB
- Volume
- 132
- Category
- Article
- ISSN
- 0022-2631
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The inside-out mode of the patch-clamp technique was used to study adenosine-5'-triphosphate (ATP)-sensitive K+ channels in mammalian skeletal muscle. Vanadate, applied to the cytoplasmic face of excised patches, was a potent activator of ATP-sensitive K+ channels. Divalent cations (Mg2+, Ca2+) were
To examine the effects of the activation of adenosine 5'-triphosphate (ATP)-sensitive K channels in a skeletal muscle we have applied the ATP-sensitive K channel opener SR44866 whilst recording single ion channels, voltage-clamped membrane currents, evoked action potentials and tension in sartorius