Blockers and inhibitors of ATP-sensitive K+channels in adult mouse skeletal muscle
β Scribed by B. Neumcke; R. Weik
- Publisher
- Springer
- Year
- 1989
- Tongue
- English
- Weight
- 116 KB
- Volume
- 414
- Category
- Article
- ISSN
- 0031-6768
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π SIMILAR VOLUMES
The inside-out mode of the patch-clamp technique was used to study adenosine-5'-triphosphate (ATP)-sensitive K+ channels in mammalian skeletal muscle. Vanadate, applied to the cytoplasmic face of excised patches, was a potent activator of ATP-sensitive K+ channels. Divalent cations (Mg2+, Ca2+) were
Patch-clamp techniques were used to study the effects of internal nucleotide diphosphates on the KAT e channel in mouse skeletal muscle. In inside-out patches, application of GDP (100 gM) and ADP (100 gM) reversibly increased the channel activity. In the presence of internal Mg 2+ (1 mM), low concen