To inspect the effect of main MS analysis conditions and data accuracy through matrix-assisted laser desorption ionization-time of flight-mass spectrometry and to obtain peptides mass fingerprint (PMF) for protein search results. BSA had been separated by SDS-PAGE, hydrolyzed by trypsin, and identif
Visualization procedures for proteins and peptides on flat-bed monoliths and their effects on matrix-assisted laser-desorption/ionization time-of-flight mass spectrometric detection
β Scribed by Wouters, Bert; Vanhoutte, Dominique J.D.; Aarnoutse, Petra; Visser, Adriaan; Stassen, Catherine; Devreese, Bart; Kok, Wim Th.; Schoenmakers, Peter J.; Eeltink, Sebastiaan
- Book ID
- 121827563
- Publisher
- Elsevier Science
- Year
- 2013
- Tongue
- English
- Weight
- 837 KB
- Volume
- 1286
- Category
- Article
- ISSN
- 1873-3778
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Studies have been made on the influence of matrix concentration and the selection of matrix-solvent combinations for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS). Sodium polystyrene sulfonate (SPS) with five different degrees of polymerization, M w = 164
Fundamental concepts of high-resolution mass spectrometry as it relates to the issue of accurate mass measurements are presented. Important issues speciΓc to magnetic sector instruments, Fourier transform ion cyclotron resonance and time-of-Γight (TOF) methods are discussed. Recent high-resolution T
A series of hexa-to decapeptides (molecular mass range 800-1200) were labeled with naphthalene-2,3dicarboxaldehyde, which preferentially reacts with the primary amino groups of a peptide. A highly stable peptide conjugate is formed, which allows selective analysis by Γuorescence at excitation and em
A rapid methodology is described for the enhancement of the signal-to-base-line (S/B) ratio of high molecular weight protein signals from whole cell bacteria analyzed by matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI-TOFMS). The procedure involves depositing gro