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On-probe sample pretreatment for the detection of proteins above 15 KDa from whole cell bacteria by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

✍ Scribed by Angelo J. Madonna; Franco Basile; Imma Ferrer; Mohammed A. Meetani; Jon C. Rees; Kent J. Voorhees

Publisher: John Wiley and Sons
Year: 2000
Tongue: English
Weight: 663 KB
Volume: 14
Category: Article
ISSN: 0951-4198
DOI: 10.1002/1097-0231(20001215)14:23<2220::aid-rcm155>3.0.co;2-4

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✦ Synopsis

A rapid methodology is described for the enhancement of the signal-to-base-line (S/B) ratio of high molecular weight protein signals from whole cell bacteria analyzed by matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI-TOFMS). The procedure involves depositing growing bacteria colonies from culture dishes directly onto the MALDI probe followed by treatment of the sample spot with a 2 mL aliquot of 40% ethanol prior to the addition of a ferulic acid matrix solution (12.5 mg dissolved in 17% formic acid/33% acetonitrile/50% H 2 O). Protein signals of more than 20 kDa were routinely produced from both Gram positive and Gram negative bacteria prepared in this manner. Moreover, a substantial number of intense protein signals were also produced in the more 'conventional' fingerprint region extending from 4 to 20 kDa. This approach is rapid, easy to implement into existing methodologies, and does not require any special hardware.

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