✦ LIBER ✦

Rapid profiling of E. coli proteins up to 500 kDa from whole cell lysates using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

✍ Scribed by Bathsheba E. Chong; Daniel B. Wall; David M. Lubman; Shannon J. Flynn

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 193 KB
Volume: 11
Category: Article
ISSN: 0951-4198
DOI: 10.1002/(sici)1097-0231(199711)11:17<1900::aid-rcm95>3.0.co;2-k

No coin nor oath required. For personal study only.

✦ Synopsis

Matrix

-assisted laser desorption/ionization time-of-flight mass spectrometry was used to rapidly detect and profile large proteins from Escherichia coli whole cell lysates in the mass range 25-500 kDa. The bacterial samples were treated with guanidine hydrochloride and Triton X-100 to disrupt and solubilize the large inner membrane proteins. A sample preparation involving a nitrocellulose polymer film, and α-cyano-4hydroxycinnamic acid, sinapinic acid or caffeic acid as matrix was utilized to rapidly monitor the presence of induced and repressed protein synthesis in response to L-arabinose catabolism in E. coli cells. The results were compared to those of 1-D or 2-D gel electrophoresis.

📜 SIMILAR VOLUMES

Fingerprint matching of E.coli strains w

Fingerprint matching of E.coli strains with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of whole cells using a modified correlation approach

✍ Randy J. Arnold; James P. Reilly 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 137 KB 👁 1 views

Protein profiles and identification of h

Protein profiles and identification of high performance liquid chromatography isolated proteins of cancer cell lines using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

✍ Bathsheba E. Chong; David M. Lubman; Allen Rosenspire; Fred Miller 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 119 KB 👁 2 views

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) has been used to rapidly profile the protein content of human cell lysates from MCF-10 cell and variant lines. The method was used to study the protein profiles of these cells as they progressed from normal br

Rapid screening of protein profiles of h

Rapid screening of protein profiles of human breast cancer cell lines using non-porous reversed-phase high performance liquid chromatography separation with matrix-assisted laser desorption/ionization time-of-flight mass spectral analysis

✍ Bathsheba E. Chong; David M. Lubman; Fred R. Miller; Allen J. Rosenspire 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 69 KB 👁 2 views

Non-porous reversed-phase (NP-RP) HPLC has been used to rapidly generate protein profiles of whole cell lysates of human breast cancer cell lines. The non-porous packing material used was silica coated with C18, which provided rapid separation with high collection efficiency of proteins from cell ly

Web-based two-dimensional database of Sa

Web-based two-dimensional database of Saccharomyces cerevisiae proteins using immobilized pH gradients from pH 6 to pH 12 and matrix-assisted laser desorption/ionization-time of flight mass spectrometry

✍ Robert Wildgruber; Gerold Reil; Oliver Drews; Harun Parlar; Angelika Görg 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 139 KB

An image based two-dimensional (2-D) reference map of very alkaline yeast cell proteins was established by using immobilized pH gradients (IPG) up to pH 12 (IPG 6-12, IPG 9-12 and IPG 10-12) for 2-D electrophoresis and by using matrix-assisted laser desorption/ionization-time of flight mass spectrom

Rapid identification and screening of pr

Rapid identification and screening of proteins from whole cell lysates of human erythroleukemia cells in the liquid phase, using non-porous reversed phase high-performance liquid chromatography separations of proteins followed by multi-assisted laser desorption/ionization mass spectrometry analysis and sequence database searching

✍ Yajuan Chen; Dan Wall; David M. Lubman 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 97 KB 👁 2 views

Non-porous reversed phase (NPRP) high-performance liquid chromatography (HPLC) has been used as a rapid method to separate proteins from whole cell lysates of human erythroleukemia (HEL) cells. Using phosphate-buffered saline (PBS) as a lysis buffer to extract proteins from HEL cells, more than 100