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Versatile vectors for transient and stable expression of recombinant antibody molecules in mammalian cells

✍ Scribed by Lars Norderhaug; Tove Olafsen; Terje E Michaelsen; Inger Sandlie

Book ID
114051764
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
256 KB
Volume
204
Category
Article
ISSN
0022-1759

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New type of plasmids named pEdHCG1 and pEdHC~:.were constructed. A combination of two plasmids containing the amplified gene product for the variable region of the antibody (Ab) heavy or light chains by the polymerase chain reaction (PCR) was co-transfected into COS cells to transiently express and