✦ LIBER ✦

A new system for the expression of recombinant antibody in mammalian cells

✍ Scribed by Yoshitaka Iba; Takashi Kaneko; Teiji Ekida; Kenji Miyata; Kuniyo Inouye; Yoshikazu Kurosawa; Kiyoshi Yasukawa

Publisher: Springer Netherlands
Year: 1995
Tongue: English
Weight: 336 KB
Volume: 17
Category: Article
ISSN: 0141-5492
DOI: 10.1007/bf00127976

No coin nor oath required. For personal study only.

✦ Synopsis

New type of plasmids named pEdHCG1 and pEdHC~:.were constructed. A combination of two plasmids containing the amplified gene product for the variable region of the antibody (Ab) heavy or light chains by the polymerase chain reaction (PCR) was co-transfected into COS cells to transiently express and in Chinese hamster ovary (CHO) cells to constitutively express chimeric Ab composed of the mouse-derived variable region and human-derived constant region.

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