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V(D)J recombinase-mediated deletion of the hprt gene in T-lymphocytes from adult humans

✍ Scribed by James C. Fuscoe; Lisa J. Zimmerman; Karen Harrington-Brock; Lisa Burnette; Martha M. Moore; Janice A. Nicklas; J.Patrick O'Neill; Richard J. Albertini

Book ID: 113255698
Publisher: Elsevier Science
Year: 1992
Weight: 575 KB
Volume: 283
Category: Article
ISSN: 0165-7992
DOI: 10.1016/0165-7992(92)90116-y

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V(D)J recombinase is normally involved in the highly nested PCR with two sets of primers. Multiple repliregulated rearrangement of immunoglobulin and T-cates of genomic DNA (each representing 4 1 10 5 cell-receptor gene segments (in B and T cells, re-cells) are amplified with specific primers under

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