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Quantification of illegitimate V(D)J recombinase-mediated mutations in lymphocytes of newborns and adults

โœ Scribed by Julia B Scheerer; Liqiang Xi; Geremy W Knapp; R.Woodrow Setzer; William L Bigbee; James C Fuscoe


Book ID
114072619
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
259 KB
Volume
431
Category
Article
ISSN
0027-5107

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V(D)J recombinase is normally involved in the highly nested PCR with two sets of primers. Multiple repliregulated rearrangement of immunoglobulin and T-cates of genomic DNA (each representing 4 1 10 5 cell-receptor gene segments (in B and T cells, re-cells) are amplified with specific primers under