Optimized C18 reversed-phase systems for oxytocin, desamino-oxytocin, lysine-vasopressin, ornithine-vasopressin and felypressin with gradient elution are discussed, focussing on precision, selectivity and ruggedness of the methods. Data from collaborative studies are presented, demonstrating the equ
Vasopressin, oxytocin and synthetic analogues: The use of bioassays
✍ Scribed by E. Stürmer
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 528 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0731-7085
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✦ Synopsis
Bioassay procedures have been the prerequisite for detection, purification, elucidation of the structure and the synthesis of neurohypophysial hormones. After a review of the history of these bioassays and of the standard preparations including international standards, the current international standards and the bioassay methods prescribed by the pharmacopoeias are described. Some important methodological details are also mentioned.
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## Abstract Blood concentrations of oxytocin (OT), vasopressin (VP) and their associated neurophysins were measured before and after the first electroconvulsive therapy (ECT) given to 12 depressed patients. The maximum concentrations of all four peptides coincided at +2 min, but the increases in OT