𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Variation in the size of human apolipoprotein(a) is due to a hypervariable region in the gene

✍ Scribed by Gisela Lindahl; Elisabeth Gersdorf; Hans Jürgen Menzel; Mary Seed; Steve Humphries; Gerd Utermann

Publisher
Springer
Year
1990
Tongue
English
Weight
551 KB
Volume
84
Category
Article
ISSN
0340-6717

No coin nor oath required. For personal study only.

✦ Synopsis

We have investigated whether the size heterogeneity of the human apolipoprotein(a) [apo(a)] is due to differences in the number of plasminogen kringle 4-like repeat units present in the different alleles. Using the Southern blot hybridization technique and a DNA probe for the kringle 4 domain of plasminogen, we have observed that in 31 different individuals a 5.8-kb PvuII restriction fragment band varies widely in intensity relative to other bands. A strong correlation (r = 0.76, P less than 0.001) was found between apo(a) protein size and the variation in intensity of the detected restriction fragment band. We confirmed this correlation in a large family where the parents are heterozygous for the apo(a) protein size isoforms. The specificity of the 5.8-kb band was established by using an apo(a)-specific oligonucleotide. These correlations strongly suggest that the observed size heterogeneity in apo(a) protein is due to different numbers of copies of the kringle 4 sequence in the apo(a) glycoprotein gene.

📜 SIMILAR VOLUMES

Variation in the hepatitis C virus NS5a
Variation in the hepatitis C virus NS5a region in relation to hypervariable region 1 heterogeneity during interferon treatment
✍ Odeberg, Jacob; Yun, Zhibing; Sönnerborg, Anders; Weiland, Ola; Lundeberg, Joaki 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 146 KB 👁 1 views

The putative interferon sensitivity determining region (ISDR) in the NS5a region of the hepatitis C virus (HCV) was analyzed in 13 interferon alpha (IFN-␣) treated patients representing genotypes 1a, 1b, and 2b. These patients had previously been followed longitudinally during treatment with respect

Guanidine to adenine (G/A) substitution
Guanidine to adenine (G/A) substitution in the promoter region of the apolipoprotein AI gene is associated with elevated serum apolipoprotein AI levels in chinese non-smokers
✍ Dr. N. Saha; J. S. H. Tay; P. S. Low; S. E. Humphries 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 627 KB

The influence of the guanidine to adenine (G/A) substitution in the promoter region of the apolipoprotein (apo) A1 gene (at -75 bp) on serum lipids and apolipoproteins was studied in 287 healthy Chinese of both sexes in Singapore. Women had significantly higher levels of high-density lipoprotein cho

The apolipoprotein(a) gene resides on hu
The apolipoprotein(a) gene resides on human chromosome 6q26–27, in close proximity to the homologous gene for plasminogen
✍ Susan Lynn Frank; Ivana Klisak; Robert S. Sparkes; T. Mohandas; James E. Tomlins 📂 Article 📅 1988 🏛 Springer 🌐 English ⚖ 618 KB

Apolipoprotein(a) [apo(a)], the glycoprotein associated with the lipoprotein(a) [Lp(a)] subfraction of plasma lipoproteins, has been shown to exhibit heritable molecular weight isoforms ranging from 400-700 kDa. Increased serum concentrations of Lp(a) correlate positively with the risk of atheroscle

An Ala/Thr variation in the coding regio
An Ala/Thr variation in the coding region of the human cystatin C gene (CST3) detected as a SstII polymorphism
✍ Milagros Balbín; Anders Grubb; Magnus Abrahamson 📂 Article 📅 1993 🏛 Springer 🌐 English ⚖ 157 KB

A DNA variation in the coding region of the human cystatin C gene has been detected by direct sequencing. The polymorphism, a G/A transition, leads to an Ala/Thr variation in the penultimate amino acid of the signal peptide. The base substitution results in the loss of a SstII restriction site, thus