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An Ala/Thr variation in the coding region of the human cystatin C gene (CST3) detected as a SstII polymorphism

✍ Scribed by Milagros Balbín; Anders Grubb; Magnus Abrahamson


Publisher
Springer
Year
1993
Tongue
English
Weight
157 KB
Volume
92
Category
Article
ISSN
0340-6717

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✦ Synopsis


A DNA variation in the coding region of the human cystatin C gene has been detected by direct sequencing. The polymorphism, a G/A transition, leads to an Ala/Thr variation in the penultimate amino acid of the signal peptide. The base substitution results in the loss of a SstII restriction site, thus allowing the design of a rapid polymerase chain reaction assay for analysis of this polymorphism in the population.