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Variation in the hepatitis C virus NS5a region in relation to hypervariable region 1 heterogeneity during interferon treatment

✍ Scribed by Odeberg, Jacob; Yun, Zhibing; Sönnerborg, Anders; Weiland, Ola; Lundeberg, Joakim

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
146 KB
Volume
56
Category
Article
ISSN
0146-6615

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✦ Synopsis

The putative interferon sensitivity determining region (ISDR) in the NS5a region of the hepatitis C virus (HCV) was analyzed in 13 interferon alpha (IFN-␣) treated patients representing genotypes 1a, 1b, and 2b. These patients had previously been followed longitudinally during treatment with respect to viral load and to virus heterogeneity using the hypervariable region 1 (HVR1) sequence as a marker. In the present study, the NS5a region was analyzed for nonresponders and sustained responders using direct DNA sequencing. While the previous results of analyzing viral composition and load showed evidence of selection, no corresponding selection of specific NS5a ISDR sequences was observed in the nonresponders, and identical ISDR sequences were observed among both sustained responders and nonresponders. Thus, we cannot verify a correlation between ISDR sequence and the observed selection of IFN-␣resistant quasispecies demonstrated as a restriction of HVR1 heterogeneity. This indicates that the potential for using ISDR as a diagnostic or prognostic marker during IFN-␣ treatment is limited.

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