Treatment of Friend-erythroleukemia cells with 1.5% dimethylsulfoxide (DMSO) caused a decrease in ouabain sensitive 86Rb+-uptake beginning six to seven hours after DMSO addition indicating a reduced function of the Na+, K+-pump. However, analysis of the ouabain sensitive 86Rb+-uptake after Na+-prelo
Vanadium Stimulates the ( Na+, K+) Pump in Friend Erythroleukemia Cells and Blocks Erythropoiesis
β Scribed by Leigh H. English, Ian G. Macara and Lewis C. Cantley
- Book ID
- 124787620
- Publisher
- Rockefeller University Press.
- Year
- 1983
- Tongue
- English
- Weight
- 735 KB
- Volume
- 97
- Category
- Article
- ISSN
- 0021-9525
- DOI
- 10.2307/1610549
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The growth of an epithelial canine kidney line (MDCK) was reversibly arrested by gradually lowering t h e serum concentration in the medium over a 3-day period. The cells were demonstrably quiescent by autoradiography after an additional 24 hours in serum-free media. Addition of fresh serum produced
## Abstract Addition of (Arg) vasopressin to quiescent cultures of Swiss 3T3 cells rapidly stimulates an ouabainβsensitive ^86^Rb uptake. In contrast the hormone has no significant effect on the rate of efflux of this cation from preloaded cells. The stimulation of ^86^Rb uptake is cycloheximideβin