Use of Vapor-Phase Acid Hydrolysis for Mass Spectrometric Peptide Mapping and Protein Identification
β Scribed by Gobom, Johan; Mirgorodskaya, Ekaterina; Nordhoff, Eckhard; Hojrup, Peter; Roepstorff, Peter
- Book ID
- 127107923
- Publisher
- American Chemical Society
- Year
- 1999
- Tongue
- English
- Weight
- 191 KB
- Volume
- 71
- Category
- Article
- ISSN
- 0003-2700
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Capillary electrophoresis/electrospray ionization (CE/ESI) high mass accuracy time-of-flight mass spectrometry was used for the first time to characterize small proteins using peptide mapping. To identify small proteins, the intact proteins were first analyzed to obtain their average molecular weigh
## Abstract Mass spectrometry (MS) together with genome database searches serves as a powerful tool for the identification of proteins. In proteome analysis, mixtures of cellular proteins are usually separated by sodium dodecyl sulfate (SDS) polyacrylamide gelβbased twoβdimensional gel electrophore