✦ LIBER ✦

Use of the metal-ion indicator, Arsenazo III, in the measurement of calcium binding

✍ Scribed by W.B. Gratzer; G.H. Beaven

Publisher: Elsevier Science
Year: 1977
Tongue: English
Weight: 688 KB
Volume: 81
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(77)90604-2

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✦ Synopsis

The use of the metal-ion indicator, Arsenazo III. for the spectrophotometric measurement of total and free calcium concentrations has been explored. The indicator can be used satisfactorily in the physiological pH range. A procedure is described for the direct measurement of calcium-binding equilibria in a ternary system containing the indicator, calcium ions. and a calcium-binding macromolecule. This appears to offer some advantages over conventional methods in situations in which the total calcium-binding capacity is low. Results on two proteins are reported, and the limitations of the method are discussed.

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