Bovine α-Lactalbumin: Identification of two metal-ion-binding sites using the europium (III) luminescent probe

Two-dimensional 1H NMR studies of Ca(II)

Two-dimensional 1H NMR studies of Ca(II)-binding site in proteins using paramagnetic lanthanides(III) as probes and Yb(III)-substituted bovine α-lactalbumin as an example

✍ Li-june Ming 📂 Article 📅 1993 🏛 John Wiley and Sons 🌐 English ⚖ 581 KB

## Abstract Substitution of Yb^3+^ for the Ca^2+^ in bovine α‐lactalbumin affords a derivative that gives rise to several isotropically shifted ^1^H NMR signals in the range 80 to − 35 ppm in D~2~O. Despite the broadness of the isotropically shifted features, cross signals in both NOESY and COSY sp