Multilayer membranes composed of avidin and biotin-labeled glucose oxidase (B-GOD) or of an avidin-B-GOD complex and B-GOD were prepared on an indium tin oxide (ITO) electrode, based on the high affinity between avidin and B-GOD. The B-GOD-modified ITO electrodes can be used to determine glucose ele
Use of the avidin-biotin system for immobilization of an enzyme on the electrode surface
β Scribed by Jun-ichi Anzai; Tomonori Hoshi; Shouryu Lee; Tetsuo Osa
- Book ID
- 108028822
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 234 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0925-4005
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A procedure is given for the preparation of an avidin monomer affinity column which is useful in the purification of biotin-containing enzymes. Both the propionyl-CoA carboxylase of Mycobacterium smegmatis and the methylmalonyl-CoA pyruvate transcarboxylase (EC 2.1.3.1) ofArachniapropionica and Prop
## Abstract Horseradish peroxidase (HRP) is immobilized in three easy steps on SiO~2~ surfaces with the help of a polycationic second generation dendronized polymer (denpol) and the biotinβavidin system. This stepwise immobilization process is monitored and quantitatively analyzed with the transmis