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Preparation of enzyme multilayers on electrode surface by use of avidin and biotin-labeled enzyme for biosensor applications

✍ Scribed by Pin-Gang He; Takeo Takahashi; Tomonori Hoshi; Jun-ichi Anzai; Yasuo Suzuki; Tetsuo Osa

Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
315 KB
Volume
2
Category
Article
ISSN
0928-4931

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✦ Synopsis

Multilayer membranes composed of avidin and biotin-labeled glucose oxidase (B-GOD) or of an avidin-B-GOD complex and B-GOD were prepared on an indium tin oxide (ITO) electrode, based on the high affinity between avidin and B-GOD. The B-GOD-modified ITO electrodes can be used to determine glucose electrochemically. The magnitude of the output signal (i.e., electric current) of the electrodes depended clearly on the number of enzyme layers.

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