An avidin monomer affinity column for the purification of biotin-containing enzymes

✍ Scribed by Katherine P. Henrikson; S.H.George Allen; W.Lee Maloy

• Publisher: Elsevier Science
• Year: 1979
• Tongue: English
• Weight: 316 KB
• Volume: 94
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(79)90374-9

✦ Synopsis

A procedure is given for the preparation of an avidin monomer affinity column which is useful in the purification of biotin-containing enzymes. Both the propionyl-CoA carboxylase of Mycobacterium smegmatis and the methylmalonyl-CoA pyruvate transcarboxylase (EC 2.1.3.1) ofArachniapropionica and Propionibacterium shermanii bind to the column and can be specifically eluted with (+)-biotin.