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Use of outer membrane protein PhoE as a carrier for the transport of a foreign antigenic determinant to the cell surface of Escherichia coli K-12

✍ Scribed by Agterberg, Marja; Adriaanse, Henriëtte; Tommassen, Jan


Book ID
122477685
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
578 KB
Volume
59
Category
Article
ISSN
0378-1119

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Gene encoding a hybrid OmpF-PhoE pore pr
✍ Tommassen, Jan ;Pugsley, Anthony P. ;Korteland, Jaap ;Verbakel, John ;Lugtenberg 📂 Article 📅 1984 🏛 Springer 🌐 English ⚖ 685 KB

To study the structure-function relationship of outer membrane pore proteins of E. coli K12, a hybrid gene was constructed in which the DNA encoding amino acid residues 2-73 of the mature PhoE protein is replaced by the homologous part of the related ompF gene. The product of this gene is incorporat