Alkaline hydrolysis of protein followed by reaction with o-phthalaldehyde has permitted the determination of less than 10 ng of protein fluorometrically. When intact proteins were analyzed with o-phthalaldehyde the reaction was less sensitive. This reaction has been applied to analysis of the protei
Use of o-phthalaldehyde assay to determine protein contents of Alhydrogel-based vaccines
โ Scribed by Daming Zhu; Allan Saul; Shuhui Huang; Laura B. Martin; Louis H. Miller; Kelly M. Rausch
- Book ID
- 116961855
- Publisher
- Elsevier Science
- Year
- 2009
- Tongue
- English
- Weight
- 372 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0264-410X
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Fluorometric procedures were developed to permit measurement of total protein concentration in nanoliter serum samples, using either fluorescamine or ophthalaldehyde. The sensitivities of assays using these two reagents were similar, but the o-phthalaldehyde method was found to be somewhat simpler a
The bicinchoninic acid (BCA) assay method for the determination of protein has been investigated for its utility in measuring the protein content of plasma lipoproteins. Although other methods, principally those based on the method of Lowry et al. (1951, J. Biol. Chem. 193, 265-275) have been extens
Quantitation of small tissue samples for total protein content is essential for many biochemical analyses. In this study a ninhydrin method for measuring the total protein content of tissue hydrolysates is presented. The ninhydrin reagent is stable at room temperature for up to 1 month in the ethyle