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Use of HPLC for increasing the molar specific activities of tritium-labelled nucleosides

✍ Scribed by B. K. Kudelin; L. V. Gavrilina; Yu. L. Kaminski

Publisher: John Wiley and Sons
Year: 1992
Tongue: French
Weight: 250 KB
Volume: 31
Category: Article
ISSN: 0022-2135
DOI: 10.1002/jlcr.2580310912

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✦ Synopsis

Abstract

The possibility of chromatographic separation of different isotopic forms of tritiated nucleosides has been demonstrated. Using a reversed‐phase column in 3–5% solutions of ethanol in water the labelled nucleosides eluted ahead of corresponding unlabelled analogs. Such isotope effects allow simultaneous fine purification of nucleosides and its fractionation according to molar specific activity value.

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